|
KMID : 0358420080510010073
|
|
Korean Journal of Obstetrics and Gynecology
2008 Volume.51 No. 1 p.73 ~ p.81
|
|
|
Dexamethasone effects on survival, differentiation, and proliferation of peritoneal (or B-1 cells) and splenic B cells
|
|
Choi Joo-Hyuk
Jung In-Cheul
Park Dong-Choon
|
|
|
Abstract
|
|
|
Objective : Although the characteristic feature and function of peritoneal B (B-1) cells are very different from splenic B (B-2) cells, peritoneal B cell study is not known well. The aim of this study is to investigate the effects of dexamethasone on peritoneal (or B-1 cells) and splenic B cells (or B-2 cells).
Methods : The synthetic corticosteroid, dexamethasome, was injected to BALBc mice intraperitoneally or subcutaneously at 4-5 pm for 7 days. Expression level of B cell surface marker analyzed by flow cytometry. The purified peritoneal B cell and splenic B cells were obtained and cell survival rate was analysed by flow cytometry. Isolated B cells were cultured in medium with different concentration of dexamaethasone. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. Entering of S phase was measured by proliferative assay.
Results : Subcutaneous injection of high dose of Dexamethasone for 7 days did not affect cell surface markers of peritoneal and splenic B cells. However, all cell surface markers of peritoneal B cell after the treatment of dexamethasone are reduced by daily intraperitoneal injection of dexamethasone for 2 days. The survival rate of peritoneal and splenic B cell decrease with increasing concentration of dexamethasone. Proliferation of peritoneal B cells was less affected by dexamethasone than that of splenic B cells (p<0.05).
Conclusion : Dexamethasone induced apoptosis in both peritoneal and splenic B cells. Proliferation and differentiation of splenic B cells were affected by dexamethasone, but peritoneal B cells are less sensitive to dexamethasone.
|
|
|
KEYWORD
|
|
|
Dexamethasone, Peritoneal B cell, Splenic B cell
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|